Biotechnological potential of marine microorganisms

Pré-requis nécessaires

Basic knowledge on microbial structure, growth and physiology (practical and theoretical)

Compétences visées

At the end of this course, students will:

-be aware of the huge diversity of marine heterotrophic micro-organisms

-understand the link between biodiversity, microbial physiology and potential biotechnological applications

-have a basic knowledge on the use of molecular tools for biotechnological applications

-have a thorough knowledge of innovative approaches used for the isolation and culture of marine heterotrophic microorganisms

-be aware of the classically used screening methods

-know the basic principles of biomass and metabolites production in bioreactors

-have examples of research applications for the industries with the use of marines microorganisms or metabolites

Descriptif

Chapter 1: Presentation of the huge diversity of heterotrophic marine microorganisms focusing on the link between biodiversity (adaptation to different habitats, metabolism, communication/interaction..) and their potential biotechnological applications (4h G Le Blay)

Chapter 2 : Presentation of innovative approaches used for marine heterotrophic microorganism isolation (dilution to extinction, micro-encapsulation, optical tweezers, diffusion chambers etc..) and culture (high throughput cultural techniques and design of culture media) (4h G Le Blay)

Chapter 3 : Screening methodologies (cultural and molecular techniques) used for industrial targets (antimicrobial, antifouling, polyhydroxyalkanoate...) (4h C Hellio)

Chapter 4 :  Presentation of the basic principles of marine microbial biomass and metabolite production (bioreactor design and operation modes,  yields and stoichiometry) (4h G Le Blay)

Chapter 5 : Presentation of examples of research applications for the industries with the use of marines microorganisms or metabolites (biofouling, -Presentation of examples of research applications for the industries with the use of marines microorganisms or metabolites (biofouling, exopolysaccharides, polyhydroxyalkanoates..) (8h C Hellio, C Simon-Colin)

Chapter 6 : Presentation of molecular tools (data mining..) for biotechnological applications (8h M Jebbar, G Burgaud, L Meslet )

Bibliographie

New approaches for bringing the uncultured into culture. S L’Haridon, GH Markx, CJ Ingham, L Paterson, F Duthoit & G Le Blay. In The marine microbiome – an untold resource of biodiversity and biotechnological potential Editors: L.J. Stal & M.S. Cretoiu Publisher: Springer 2016

Screening microorganisms for bioactive compounds. S Giubergia, C Schleissner, F de la Calle, Pretsch, D Pretsch, L Gram & MS Thøgersen. In The marine microbiome – an untold resource of biodiversity and biotechnological potential Editors: L.J. Stal & M.S. Cretoiu Publisher: Springer 2016

Exploring the microbiology of the deep sea. M Jebbar, P Vannier, G Michoud & VT

Marteinsson. In The marine microbiome – an untold resource of biodiversity and biotechnological potential Editors: L.J. Stal & M.S. Cretoiu Publisher: Springer 2016

 

Marine fungi. V Rédou, M Vallet, L Meslet-Cladière, A Kumar, KL Pang, YF Pouchus, G Barbier, O Grovel, S Bertrand, S Prado, C Roullier & G Burgaud. In The marine microbiome – an untold resource of biodiversity and biotechnological potential Editors: L.J. Stal & M.S. Cretoiu Publisher: Springer 2016

Entrapment of anaerobic thermophilic and hyperthermophilic marine microorganisms in a gellan/xanthan matrix. Landreau M, Duthoit F, Claeys-Bruno M, Vandenabeele-Trambouze O, Aubry T, Godfroy A, Le Blay G. J Appl Microbiol. 2016 Mar 1.

 

Discovery of a mcl-PHA with unexpected biotechnical properties: the marine environment of French Polynesia as a source for PHA-producing bacteria. Wecker P, Moppert X, Simon-Colin C, Costa B, Berteaux-Lecellier V. AMB Express. 2015 Dec;5(1):74.

 

Meslet-Cladiere, L., Delage, L., Leroux, C.J., Goulitquer, S., Leblanc, C., Creis, E., Gall, E.A., Stiger-Pouvreau, V., Czjzek, M., and Potin, P. (2013). Structure/Function analysis of a type III polyketide synthase in the brown alga Ectocarpus siliculosus reveals a biochemical pathway in phlorotannin monomer biosynthesis. Plant Cell 25, 3089-3103.

Langue d'enseignement

Anglais